Voges–Proskauer test

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Voges–Proskauer test - brief summary


Voges–Proskauer /?foÊŠÉ¡É™s ?prÉ’skaÊŠ.É™r/ or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result. Procedure: First, add the alpha-naphthol; then, add the potassium hydroxide. A reversal in the order of the reagents being added may result in a weak-positive or false-negative reaction.

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