Bacterial artificial chromosome

A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells such as E. coli.

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Use

BACs are often used in connection with DNA sequencing.

Segment length

• Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.
• The BACs, with their inserted DNA, are then taken up by bacterial cells.
• As the bacterial cells grow and divide, they amplify the BAC DNA, which can then be isolated and used in sequencing DNA.

Frequently asked questions

Chromosome-DNA-gene copy

Help answer these questions.

• How do bacterial artificial chromosomes work?

• How do you make a bacterial artificial chromosome?

• How does a bacterial artificial chromosome BAC differ from a plasmid?

• What must be present in artificial chromosome?

• What are artificial chromosomes used for?

• What is YAC and BAC?

• What are BAC transgenic mice?

• What is the F factor in bacteria?

• Is YAC a plasmid?

• What is BAC system?

• What is an advantage of BACs and YACs over plasmids?

• What is a BAC vector?

• Who is YAC?

• Is YAC a vector?

• Is YAC circular or linear?

• What are BACs and YACs How are they different?

• What is the difference between plasmid and cosmid?

• What do the sequence Tel signify in a YAC vector?

• What is the cen4 region in the YAC vector?

• What are cosmid vectors?

• How can the presence of insert DNA in the YAC vector be checked?

External links

• Wikipedia

Latest research

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• Ongoing trials

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