Dolichyl-phosphate beta-glucosyltransferase

Enzyme involved in glycosylation

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Identifiers
EC number	2.4.1.117
CAS number	62213-38-3
Alt. names
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway

Dolichyl-phosphate beta-glucosyltransferase is an enzyme that plays a crucial role in the process of glycosylation, specifically in the synthesis of glycoproteins. This enzyme is responsible for transferring a glucose molecule from UDP-glucose to dolichyl phosphate, forming dolichyl-phosphate-glucose.

Function

Dolichyl-phosphate beta-glucosyltransferase is involved in the N-linked glycosylation pathway, which is essential for the proper folding and stability of many proteins. The enzyme catalyzes the following reaction:

UDP-glucose + dolichyl phosphate → UDP + dolichyl-phosphate-glucose

This reaction is a key step in the assembly of the dolichol-linked oligosaccharide precursor, which is subsequently transferred to nascent proteins in the endoplasmic reticulum.

Structure

The structure of dolichyl-phosphate beta-glucosyltransferase has not been fully elucidated. However, it is known to be a membrane-bound enzyme located in the endoplasmic reticulum.

Clinical Significance

Mutations or deficiencies in dolichyl-phosphate beta-glucosyltransferase can lead to disorders in glycosylation, which can result in various congenital disorders of glycosylation (CDGs). These disorders often present with a wide range of symptoms, including developmental delays, neurological issues, and other systemic problems.

Related Enzymes

Dolichyl-phosphate beta-glucosyltransferase is part of a larger family of glycosyltransferases, which are enzymes that facilitate the transfer of sugar moieties to various substrates. Other related enzymes include:

• Dolichyl-phosphate-mannose synthase
• Dolichyl-phosphate-glucose synthase

See Also

• Glycosylation
• Endoplasmic reticulum
• Congenital disorders of glycosylation
• Glycoprotein