Isoluminol

Isoluminol is a chemical compound used in various biochemical assays due to its chemiluminescence properties. It is a derivative of luminol, and is often used in immunoassays and DNA sequencing due to its high sensitivity and broad dynamic range.

Chemical Structure

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Chemical structure of Isoluminol

Isoluminol has a chemical formula of C8H7N3O2. It is a heterocyclic compound with two benzene rings connected by a nitrogen atom. The molecule also contains two hydroxyl groups and an amino group.

Chemiluminescence

Isoluminol is known for its chemiluminescent properties. When oxidized in the presence of a catalyst, it emits light. This property is utilized in various biochemical assays, including immunoassays and DNA sequencing.

Applications

Immunoassays

In immunoassays, isoluminol is often used as a label for antibodies. The chemiluminescent reaction is triggered when the antibody binds to its target, allowing for the detection and quantification of the target molecule.

DNA Sequencing

Isoluminol can also be used in DNA sequencing. The chemiluminescent reaction is triggered when a specific DNA sequence is present, allowing for the detection and identification of the sequence.

Safety

As with all chemicals, isoluminol should be handled with care. It is recommended to use personal protective equipment when handling isoluminol, and to follow all safety guidelines provided by the manufacturer.

See Also

• Luminol
• Chemiluminescence
• Immunoassay
• DNA sequencing