LC-MS

LC-MS or Liquid Chromatography-Mass Spectrometry is a technique used in analytical chemistry to separate, identify and quantify each component in a mixture. It combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS).

Overview

LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity. Generally its application is oriented towards the general detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture).

Principle

LC-MS system is divided into three parts. The LC module, the MS module and the computer system. Liquid chromatography separates molecules in the mixture based on their chemical properties, while mass spectrometry provides the mass of the molecules and can also identify the molecules.

Applications

LC-MS has been used in many sectors including biotechnology, environmental analysis, food safety, forensic science, and pharmaceutical industry. It is used for the detection and identification of chemicals in a complex mixture.

See also

• Gas chromatography-mass spectrometry
• High-performance liquid chromatography
• Mass spectrometry

References

LC-MS Resources
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