Pyrrolidonyl-β-naphthylamide

Pyrrolidonyl-β-naphthylamide (PβN) is a synthetic substrate used in the identification of certain bacteria. It is often used in conjunction with other tests in the process of bacterial identification.

Overview

PβN is a chromogenic substrate that is used to detect the presence of the enzyme pyrrolidonyl arylamidase (PYR). This enzyme is produced by certain species of bacteria, including some Streptococcus and Enterococcus species. The PYR test, which involves the use of PβN, is a rapid and reliable method for the identification of these bacteria.

Mechanism of Action

When PβN is added to a culture of bacteria, those that produce the PYR enzyme will break down the PβN, resulting in the release of a red-colored compound. This color change is a positive result, indicating the presence of PYR-producing bacteria.

Use in Bacterial Identification

The PYR test is a valuable tool in the identification of certain bacteria. It is particularly useful in distinguishing between different species of Streptococcus and Enterococcus, as only some of these produce the PYR enzyme. The test is simple, quick, and highly reliable, making it a valuable tool in the field of microbiology.

Safety and Handling

As with all laboratory chemicals, PβN should be handled with care. It should be stored in a cool, dry place and kept out of reach of children. Always wear appropriate personal protective equipment when handling PβN.

See Also

• Bacterial identification
• Chromogenic substrate
• Pyrrolidonyl arylamidase
• Streptococcus
• Enterococcus