Ultraviolet–visible spectroscopy

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DU640 spectrophotometer
Bis(triphenylphosphine) nickel (II) chloride UV-vis
Simplified UV-vis diagram.png

Ultraviolet–visible spectroscopy (UV-Vis spectroscopy) is a type of absorption spectroscopy in the ultraviolet and visible regions of the electromagnetic spectrum. This technique is used to measure the absorbance of light by a sample, which can provide information about the sample's chemical composition and concentration.

Principle[edit | edit source]

UV-Vis spectroscopy is based on the principle that molecules absorb light at specific wavelengths. When a molecule absorbs UV or visible light, electrons in the molecule are excited from a lower energy level to a higher energy level. The amount of light absorbed at each wavelength is measured and plotted to create an absorption spectrum.

Instrumentation[edit | edit source]

The main components of a UV-Vis spectrophotometer include:

Applications[edit | edit source]

UV-Vis spectroscopy is widely used in various fields, including:

Beer-Lambert Law[edit | edit source]

The Beer-Lambert law relates the absorbance of light to the properties of the material through which the light is traveling. The law is expressed as: \[ A = \varepsilon \cdot c \cdot l \] where:

  • \( A \) is the absorbance,
  • \( \varepsilon \) is the molar absorptivity,
  • \( c \) is the concentration of the solution,
  • \( l \) is the path length of the sample cell.

Advantages and Limitations[edit | edit source]

Advantages[edit | edit source]

  • Non-destructive analysis.
  • High sensitivity and specificity.
  • Rapid and simple to perform.

Limitations[edit | edit source]

  • Requires clear and colorless samples.
  • Limited to compounds that absorb UV or visible light.
  • Interference from other absorbing species in the sample.

See also[edit | edit source]

References[edit | edit source]

External links[edit | edit source]

Contributors: Prab R. Tumpati, MD