Acridine yellow
Acridine dye used as a fluorescent stain in biological research
Acridine yellow is a fluorescent dye belonging to the acridine family of compounds. It is primarily used as a biological stain in various microscopy techniques and fluorescence microscopy applications. Acridine yellow is known for its ability to bind to nucleic acids, making it useful in the study of DNA and RNA within cells.
Chemical Properties[edit | edit source]
Acridine yellow is a heterocyclic compound with the chemical formula C15H15N3. It is characterized by its bright yellow color and its ability to fluoresce under ultraviolet light. The compound is soluble in water and various organic solvents, which makes it versatile for use in different experimental conditions.
Applications[edit | edit source]
Acridine yellow is widely used in the field of biochemistry and molecular biology. Its primary application is as a fluorescent stain for nucleic acids. When bound to DNA or RNA, acridine yellow emits a bright fluorescence, allowing researchers to visualize and study the structure and function of genetic material within cells.
Microscopy[edit | edit source]
In fluorescence microscopy, acridine yellow is used to stain cellular structures such as the nucleus and cytoplasm. It is particularly useful in distinguishing between live and dead cells in a sample, as it can penetrate cell membranes and bind to nucleic acids.
Flow Cytometry[edit | edit source]
Acridine yellow is also employed in flow cytometry to analyze the cell cycle and apoptosis. Its ability to bind to DNA allows for the quantification of DNA content in individual cells, providing insights into cell proliferation and death.
Safety and Handling[edit | edit source]
As with many chemical dyes, acridine yellow should be handled with care. It is important to use appropriate personal protective equipment (PPE) such as gloves and goggles when working with this compound. Additionally, it should be stored in a cool, dry place away from direct sunlight to prevent degradation.
Related pages[edit | edit source]
Gallery[edit | edit source]
Acridine yellow structure
3D model of acridine yellow
Acridine yellow[edit | edit source]
Acridine yellow
Acridine yellow 3D balls
Acridine_yellow[edit | edit source]
Acridine yellow
Acridine yellow 3D balls
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