Base analog
Base analogs are compounds, usually a purine or a pyrimidine, usually fluorescent that differs somewhat from a normal nucleic acid base.
Purpose[edit | edit source]
- These analogs can be incorporated site-specifically into oligonucleotides through standard automated synthetic methods, allowing them to serve as sensitive probes of changes in the microenvironment of bases, that may result from variation in buffer condition, ion concentration, temperature or molecular interactions.
- Fluorescent nucleotide base analogs provide sensitive probes for studying the structure, dynamics and interactions of nucleic acids.
Fluorescent base analogs[edit | edit source]
Global conformational changes in nucleic acids and nucleic acid complexes can also be detected using fluorescent base analogs.
Benefits[edit | edit source]
- A significant strength of fluorescent base analogs is their similarity in molecular constitution and chemical properties to natural bases.
- In contrast to bulkier chromophores, incorporation of nucleotide base analogs into oligonucleotidescan normally be accomplished without introducing significant structural or chemical changes that might alter the measurement.
Base analog Resources | ||
---|---|---|
|
|
Translate to: East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
Urdu,
বাংলা,
తెలుగు,
தமிழ்,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
European
español,
Deutsch,
français,
русский,
português do Brasil,
Italian,
polski
Navigation: Wellness - Encyclopedia - Health topics - Disease Index - Drugs - World Directory - Gray's Anatomy - Keto diet - Recipes
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro) available.
Advertise on WikiMD
WikiMD is not a substitute for professional medical advice. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD