Base analog

Base analogs are compounds, usually a purine or a pyrimidine, usually fluorescent that differs somewhat from a normal nucleic acid base.

Purpose

• These analogs can be incorporated site-specifically into oligonucleotides through standard automated synthetic methods, allowing them to serve as sensitive probes of changes in the microenvironment of bases, that may result from variation in buffer condition, ion concentration, temperature or molecular interactions.
• Fluorescent nucleotide base analogs provide sensitive probes for studying the structure, dynamics and interactions of nucleic acids.

Fluorescent base analogs

Global conformational changes in nucleic acids and nucleic acid complexes can also be detected using fluorescent base analogs.

Benefits

• A significant strength of fluorescent base analogs is their similarity in molecular constitution and chemical properties to natural bases.
• In contrast to bulkier chromophores, incorporation of nucleotide base analogs into oligonucleotidescan normally be accomplished without introducing significant structural or chemical changes that might alter the measurement.

Base analog Resources
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