Fed-batch culture
Fed-batch culture is a semi-continuous operating mode of a bioreactor in which nutrients are added in increments as the culture progresses. This method is in contrast to batch culture, where all nutrients are provided at the start of the culture, and continuous culture, where nutrients are added and waste products removed continuously. Fed-batch culture is widely used in microbial fermentation and cell culture processes in the biotechnology industry, including the production of antibiotics, enzymes, and recombinant proteins.
Overview[edit | edit source]
In fed-batch culture, the strategy is to avoid nutrient depletion and to control the accumulation of toxic by-products by carefully feeding the culture with fresh medium. This allows for higher cell densities and product yields compared to batch culture. The feeding strategy can be based on various parameters, such as pH, dissolved oxygen, or the concentration of a specific nutrient or metabolite.
Advantages[edit | edit source]
- Increased Productivity: By avoiding nutrient limitations and toxic by-product accumulation, fed-batch cultures can achieve higher cell densities and product yields.
- Control Over Culture Conditions: The feeding strategy allows for better control over the culture environment, which can improve product quality and consistency.
- Flexibility: The feeding strategy can be adjusted based on the needs of the specific organism or product being produced.
Disadvantages[edit | edit source]
- Complexity: Fed-batch processes are more complex to design and control compared to batch cultures.
- Risk of Contamination: The addition of fresh medium increases the risk of contamination compared to a closed batch system.
- Cost: The increased complexity and monitoring requirements can lead to higher operational costs.
Applications[edit | edit source]
Fed-batch culture is used in various applications within the biotechnology industry. Some of the key applications include:
- Production of antibiotics by microbial fermentation.
- Cultivation of recombinant bacteria or yeast for the production of recombinant proteins.
- Expansion of mammalian cells for the production of monoclonal antibodies and other biopharmaceuticals.
Feeding Strategies[edit | edit source]
There are several feeding strategies used in fed-batch cultures, including:
- Constant Feeding: Nutrients are added at a constant rate throughout the culture.
- Exponential Feeding: Nutrients are added at a rate that matches the exponential growth phase of the culture.
- Pulse Feeding: Nutrients are added in discrete pulses based on the monitoring of specific culture parameters.
Conclusion[edit | edit source]
Fed-batch culture is a versatile and efficient method for the cultivation of microorganisms and cell cultures in the biotechnology industry. Its ability to achieve high cell densities and product yields, while maintaining control over the culture environment, makes it a preferred choice for many industrial applications. However, the complexity and potential risks associated with fed-batch culture require careful planning and monitoring to ensure successful outcomes.
This article is a biotechnology stub. You can help WikiMD by expanding it!
Transform your life with W8MD's budget GLP1 injections from $125 and up biweekly
W8MD offers a medical weight loss program NYC and a clinic to lose weight in Philadelphia. Our W8MD's physician supervised medical weight loss centers in NYC provides expert medical guidance, and offers telemedicine options for convenience.
Why choose W8MD?
- Comprehensive care with FDA-approved weight loss medications including:
- loss injections in NYC both generic and brand names:
- weight loss medications including Phentermine, Qsymia, Contrave, Diethylpropion etc.
- Accept most insurances for visits or discounted self pay cost.
- Generic weight loss injections starting from just $125.00 for the starting dose
- In person weight loss NYC and telemedicine medical weight loss options in New York city available
Book Your Appointment
Start your NYC weight loss journey today at our NYC medical weight loss, and Philadelphia and visit Philadelphia medical weight loss Call (718)946-5500 for NY and 215 676 2334 for PA
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's NYC physician weight loss.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available. Call 718 946 5500.
Advertise on WikiMD
WikiMD's Wellness Encyclopedia |
Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates, categories Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD