Fluorescence imaging

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Fluorescence Imaging is a technique used in biological research and medical diagnostics to visualize cellular and subcellular structures within living organisms. It uses the property of fluorescence, where certain compounds, known as fluorophores, emit light of a longer wavelength when they absorb light of a shorter wavelength.

History[edit | edit source]

The concept of fluorescence was first observed by Sir George Gabriel Stokes in 1852. However, the application of fluorescence in imaging was not realized until the 20th century, with the development of the fluorescence microscope.

Principles[edit | edit source]

Fluorescence imaging is based on the principle of fluorescence. When a fluorophore absorbs light of a specific wavelength, it gets excited to a higher energy state. After a short delay, it returns to its ground state by emitting light of a longer wavelength. This emitted light is then captured to form an image.

Techniques[edit | edit source]

There are several techniques in fluorescence imaging, including:

Applications[edit | edit source]

Fluorescence imaging has a wide range of applications in both research and clinical settings. It is used in cell biology to study the structure and function of cells, in molecular biology to study protein interactions, and in medical diagnostics to detect diseases such as cancer.

See also[edit | edit source]

References[edit | edit source]



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