IC50

From WikiMD's Wellnesspedia

IC50 is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. It is commonly used in pharmacology, biology and biochemistry. The IC50 of a drug can be determined by constructing a dose-response curve and examining the effect of different concentrations of antagonist on reversing agonist activity.

Definition[edit | edit source]

The IC50 value represents the concentration of a drug that is required for 50% inhibition in vitro. It allows for the comparison of the potency of drugs in medical research. The lower the IC50 value, the greater the potency of the drug, which means less of the drug is required to inhibit a given biological process by half.

Calculation[edit | edit source]

The IC50 value is commonly determined by constructing a dose-response curve. This curve plots the response (effect) of the drug against its concentration. The IC50 value is then determined as the concentration of the drug that gives a response halfway between the baseline and maximum after some specified exposure time.

Applications[edit | edit source]

IC50 values are used in drug discovery to identify the potency of new drugs, and can also be used in diagnosing the potential toxicity of chemicals.

Limitations[edit | edit source]

While IC50 values are useful measures of drug potency, they have several limitations. They depend on the conditions under which they are measured, including the time of exposure and the concentration of substrate.

See also[edit | edit source]

References[edit | edit source]


IC50 Resources

Find a healthcare provider anywhere in the world quickly and easily!

Wiki.png

Navigation: Wellness - Encyclopedia - Health topics - Disease Index‏‎ - Drugs - World Directory - Gray's Anatomy - Keto diet - Recipes

Search WikiMD


Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro) available.
Advertise on WikiMD

WikiMD is not a substitute for professional medical advice. See full disclaimer.

Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.


Contributors: Prab R. Tumpati, MD