Isotonic medium

An isotonic medium is a solution whose solute concentration is equal to the solute concentration inside a cell. This equilibrium in solute concentration ensures that the cell's internal environment remains stable, preventing the cell from shrinking or swelling. Isotonic solutions are crucial in medical and biological contexts, particularly in intravenous therapy and cell culture.

Properties

Osmolarity

The key property of an isotonic medium is its osmolarity, which is the measure of solute concentration. In isotonic solutions, the osmolarity is typically close to that of body fluids, approximately 300 mOsm/L.

Impact on Cells

Cells in an isotonic medium maintain their normal shape and function because there is no net movement of water into or out of the cell. This equilibrium is crucial for the survival and proper functioning of cells, especially in medical treatments and laboratory experiments.

Applications

Medical Use

In medicine, isotonic solutions like normal saline (0.9% NaCl) are used for intravenous therapy to hydrate patients or deliver medications without disturbing the body's electrolyte balance.

Laboratory Use

In laboratory settings, isotonic solutions are used to maintain cell cultures. This environment mimics the cells' natural environment, promoting health and longevity in cell lines.

Common Isotonic Solutions

• Normal saline (0.9% NaCl): Widely used in medical settings for IV hydration and medication administration.
• Lactated Ringer's solution: Contains electrolytes, mimicking the body's plasma and is often used for fluid resuscitation.
• 5% dextrose in water (D5W): Though isotonic when prepared, it becomes hypotonic in the body as glucose is metabolized.

Conclusion

Isotonic media play an indispensable role in both medical treatments and biological research. Understanding the properties and applications of isotonic solutions is essential for healthcare professionals and scientists alike.

Isotonic medium Resources
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