Van Gieson's stain

Van Gieson's Stain

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Micrograph of an angioleiomyoma stained with Van Gieson's stain.

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Histopathology of steatohepatitis with cirrhosis, showing thick bands of fibrosis stained with Van Gieson's stain.

Van Gieson's stain is a histological stain used in pathology to differentiate between collagen and other tissue components. It is particularly useful for highlighting connective tissue in microscopic sections.

Composition

Van Gieson's stain is composed of two main dyes: acid fuchsin and picric acid. The acid fuchsin stains collagen fibers red, while the picric acid stains muscle and cytoplasm yellow. This contrast allows for clear differentiation of collagen from other tissue elements.

Applications

Van Gieson's stain is commonly used in the examination of liver biopsies, especially in cases of cirrhosis and fibrosis. It is also used in the study of vascular lesions, such as angioleiomyoma, to highlight the presence of collagenous stroma.

Procedure

The staining process involves applying the Van Gieson's solution to a tissue section that has been previously stained with hematoxylin. The hematoxylin stains cell nuclei blue, providing a counterstain that enhances the visibility of the red and yellow components of the Van Gieson's stain.

Advantages and Limitations

Van Gieson's stain is valued for its simplicity and effectiveness in distinguishing collagen from other tissue types. However, it may not provide as much detail as more complex staining techniques, such as the Masson's trichrome stain.

Related Pages

• Histology
• Staining (biology)
• Connective tissue
• Cirrhosis

References

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  Angioleiomyoma with Van Gieson's stain

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  Histopathology of steatohepatitis with established cirrhosis, with thick bands of fibrosis (van Gieson)