Buffy coat
Buffy Coat
The buffy coat is a thin layer of leukocytes (white blood cells) and platelets that forms between the plasma and red blood cells in a sample of anticoagulant-treated blood that has been centrifuged. The buffy coat is typically white or light yellow in color, hence its name, which is derived from the appearance of this layer.
Composition[edit | edit source]
The buffy coat is composed primarily of white blood cells and platelets. White blood cells, also known as leukocytes, are part of the immune system and help the body fight off infections. Platelets, or thrombocytes, are small cell fragments that play a crucial role in blood clotting.
Isolation and Use[edit | edit source]
The buffy coat is often used in medical and research settings to isolate specific components of the blood. For example, it can be used to extract DNA from blood samples, or to isolate specific types of white blood cells for further study. The buffy coat can also be used in the preparation of platelet concentrate, a type of blood product used in transfusions.
Clinical Significance[edit | edit source]
An abnormal buffy coat can be indicative of various medical conditions. For example, an increased number of white blood cells in the buffy coat can be a sign of an infection or an immune response. Conversely, a decreased number of white blood cells can indicate a weakened immune system or a condition such as leukopenia. Similarly, an abnormal number of platelets can be indicative of a clotting disorder.
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