Chinese hamster ovary cell

From WikiMD.com Medical Encyclopedia

Chinese Hamster Ovary Cells[edit | edit source]

Chinese Hamster Ovary (CHO) cells in culture

Chinese Hamster Ovary (CHO) cells are a type of cell line derived from the ovary of the Chinese hamster, Cricetulus griseus. These cells are widely used in biological research and biotechnology due to their rapid growth and ability to express recombinant proteins.

History[edit | edit source]

CHO cells were first isolated in the 1950s by Dr. Theodore T. Puck and his colleagues. They have since become one of the most important mammalian cell lines used in biomedical research and pharmaceutical production.

Characteristics[edit | edit source]

CHO cells are epithelial cells that are adherent in culture. They have a relatively short doubling time, making them ideal for large-scale production. CHO cells are also known for their ability to grow in suspension cultures, which is advantageous for industrial applications.

Applications[edit | edit source]

Biotechnology[edit | edit source]

CHO cells are extensively used in the production of therapeutic proteins and monoclonal antibodies. They are preferred in the biopharmaceutical industry due to their ability to perform complex post-translational modifications, such as glycosylation, which are essential for the activity and stability of many therapeutic proteins.

Research[edit | edit source]

In academic research, CHO cells are used to study gene expression, protein folding, and cell signaling pathways. They serve as a model system for understanding the molecular biology of eukaryotic cells.

Advantages[edit | edit source]

CHO cells offer several advantages, including:

  • High growth rate and productivity
  • Ability to grow in serum-free and chemically defined media
  • Capability to perform human-like post-translational modifications
  • Well-characterized genome and availability of genetic tools

Limitations[edit | edit source]

Despite their advantages, CHO cells have some limitations:

  • Genetic instability over long-term culture
  • Potential for viral contamination
  • High cost of culture media and production

Related Pages[edit | edit source]




WikiMD
Navigation: Wellness - Encyclopedia - Health topics - Disease Index‏‎ - Drugs - World Directory - Gray's Anatomy - Keto diet - Recipes

Search WikiMD

Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD

WikiMD's Wellness Encyclopedia

Let Food Be Thy Medicine
Medicine Thy Food - Hippocrates

Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates, categories Wikipedia, licensed under CC BY SA or similar.

Retrieved from "https://wikimd.com/w/index.php?title=Chinese_hamster_ovary_cell&oldid=6300421"

Contributors: Prab R. Tumpati, MD