Collision-induced dissociation

Collision-induced dissociation

Schematic representation of collision-induced dissociation

Collision-induced dissociation (CID) is a widely used technique in mass spectrometry for fragmenting ions in order to obtain structural information about molecules. It involves subjecting ions to collisions with neutral gas molecules, resulting in their dissociation into smaller fragments. CID is particularly useful in the analysis of complex molecules, such as peptides, proteins, and small organic compounds.

Mechanism

The mechanism of collision-induced dissociation involves the transfer of kinetic energy from the colliding gas molecules to the ions. This energy transfer leads to the breaking of chemical bonds within the ions, resulting in the formation of fragment ions. The extent and pattern of fragmentation depend on various factors, including the collision energy, the nature of the gas molecules, and the structure of the ion being fragmented.

Applications

Collision-induced dissociation has numerous applications in various fields, including:

1. Proteomics: CID is extensively used in proteomics research for the identification and characterization of proteins. By subjecting protein ions to collision-induced dissociation, researchers can obtain information about the amino acid sequence and post-translational modifications of the protein.

2. Metabolomics: CID is also employed in metabolomics studies to analyze small organic molecules, such as metabolites and drugs. By fragmenting these molecules, researchers can identify their chemical structures and elucidate metabolic pathways.

3. Structural elucidation: CID is a valuable tool for determining the structure of unknown compounds. By comparing the fragmentation patterns of the unknown compound with those of known compounds, researchers can infer its structure and chemical properties.

Categories

Collision-induced dissociation falls under the following categories:

Templates

The following templates are commonly used in articles related to collision-induced dissociation:

1.

This template provides general information about mass spectrometry techniques, including collision-induced dissociation.

2.

This template is specifically designed for articles related to proteomics research, which often involves the use of collision-induced dissociation.

3.

This template is useful for articles focusing on metabolomics studies that utilize collision-induced dissociation.

Conclusion

Collision-induced dissociation is a powerful technique in mass spectrometry that allows for the fragmentation and analysis of ions. Its applications in proteomics, metabolomics, and structural elucidation have greatly contributed to our understanding of complex molecules. By utilizing internal links, categories, and templates, we can create a comprehensive and informative encyclopedia article on this topic.