H1299

H1299

H1299 is a human non-small cell lung carcinoma (NSCLC) cell line that is widely used in cancer research. This cell line is derived from the lung tissue of a patient with non-small cell lung cancer, which is the most common type of lung cancer. H1299 cells are known for their utility in studying the molecular mechanisms of lung cancer, drug resistance, and potential therapeutic targets.

Characteristics

H1299 cells are epithelial in nature and exhibit several key characteristics that make them valuable for research:

• p53 Null: H1299 cells are notable for lacking the tumor suppressor protein p53, which is a common mutation in many cancers. This makes them an important model for studying the role of p53 in cancer biology.
• Growth Properties: These cells grow adherently in culture and have a doubling time of approximately 24-36 hours under optimal conditions.
• Genetic Profile: H1299 cells have been extensively characterized genetically, providing a wealth of information for researchers studying gene expression and mutation effects.

Applications in Research

H1299 cells are used in a variety of research applications, including:

• Cancer Biology: Researchers use H1299 cells to study the pathways involved in lung cancer progression and metastasis.
• Drug Testing: These cells are used to screen potential anti-cancer drugs and to study mechanisms of drug resistance.
• Gene Editing: The absence of p53 makes H1299 cells a useful model for CRISPR-Cas9 gene editing experiments to study the effects of restoring or modifying p53 function.

Culturing H1299 Cells

Culturing H1299 cells requires specific conditions to maintain their growth and viability:

• Medium: H1299 cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics such as penicillin and streptomycin.
• Temperature and Atmosphere: The cells are maintained at 37°C in a humidified atmosphere with 5% CO2.
• Subculturing: Cells should be subcultured when they reach 70-80% confluence to prevent overgrowth and maintain healthy cell populations.

Research Findings

Studies using H1299 cells have contributed to significant findings in cancer research, such as:

• Role of p53 in Cancer: Research has shown how the absence of p53 in H1299 cells affects cell cycle regulation and apoptosis.
• Targeted Therapies: H1299 cells have been used to identify potential targets for therapy, such as specific kinases and signaling pathways involved in lung cancer.

Also see

• Non-small cell lung carcinoma
• p53 tumor suppressor
• Cancer cell line
• CRISPR-Cas9