Calu-3
Calu-3 is a human lung cancer cell line that is commonly used in scientific research. It was derived from a pleural effusion of a patient with adenocarcinoma of the lung. Calu-3 cells are known for their ability to form tight junctions and polarize, making them a valuable model for studying airway epithelium and drug transport.
Characteristics[edit | edit source]
Calu-3 cells exhibit several key characteristics that make them useful for research:
- They form tight junctions, which are crucial for maintaining the integrity of the epithelial barrier.
- They can polarize, meaning they have distinct apical and basolateral surfaces, similar to the cells lining the human respiratory tract.
- They express various cytokines and chemokines, which are important for studying inflammatory responses.
Applications in Research[edit | edit source]
Calu-3 cells are widely used in various fields of research, including:
- Respiratory disease studies: Due to their origin and characteristics, Calu-3 cells are used to model diseases such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease (COPD).
- Drug delivery and pharmacokinetics: Researchers use Calu-3 cells to study the transport and absorption of drugs across the airway epithelium.
- Virology: Calu-3 cells are used to study the infection mechanisms of various respiratory viruses, including influenza and coronavirus.
Culturing Calu-3 Cells[edit | edit source]
Calu-3 cells are typically cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (FBS). They require a humidified atmosphere with 5% carbon dioxide (CO2) at 37°C. Proper maintenance of these cells is crucial for ensuring their viability and functionality in experiments.
See Also[edit | edit source]
References[edit | edit source]
External Links[edit | edit source]
Navigation: Wellness - Encyclopedia - Health topics - Disease Index - Drugs - World Directory - Gray's Anatomy - Keto diet - Recipes
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
WikiMD is not a substitute for professional medical advice. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Contributors: Prab R. Tumpati, MD