HNCA experiment

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== HNCA Experiment ==

The HNCA experiment, short for Heteronuclear Single Quantum Coherence with Nitrogen Carbon Alpha detection, is a widely used NMR spectroscopy technique in structural biology. It is particularly valuable in the study of proteins and peptides.

Principle[edit | edit source]

In the HNCA experiment, the nitrogen atom (N) is used as the heteronuclear nucleus, while the carbon atom (C) attached to the alpha carbon of the amino acid is detected. This experiment provides valuable information about the backbone of proteins and peptides.

Procedure[edit | edit source]

During the HNCA experiment, a series of pulses and delays are applied to the sample. These manipulations allow for the transfer of magnetization between the nitrogen and carbon nuclei, leading to the detection of the desired signals.

Applications[edit | edit source]

The HNCA experiment is commonly used in protein structure determination studies, as it provides crucial information about the connectivity of amino acids in the protein sequence. By analyzing the data obtained from the HNCA experiment, researchers can deduce the sequential order of amino acids in the protein.

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v t e Nuclear Magnetic Resonance (NMR) Spectroscopy
Fundamentals	NMR Basics * Chemical shift * Spin–spin coupling * NOE * Relaxation
Techniques	1D NMR * 2D NMR COSY HSQC HMBC NOESY * 3D NMR * 4D NMR * Solid-state NMR * Surface-enhanced NMR
Applications	Proteins * Nucleic acids * Metabolomics * Small-molecule NMR
Hardware	NMR magnet * Cryoprobe * Magic angle spinning * NMR spectrometer
Software	NMR data processing * NMR spectrum prediction
Related topics	MRI * EPR * Mössbauer spectroscopy
This NMR spectroscopy related article is a stub.

v t e Proteins
Basics	Amino acid Peptide bond Primary structure Secondary structure Tertiary structure Quaternary structure Protein folding Protein structure prediction Protein–protein interaction
Types	Enzyme Hormone Structural protein Motor protein Transport protein Storage protein Signal protein Receptor protein Defensive protein Gene regulatory protein Special-purpose proteins
Techniques	Protein purification X-ray crystallography NMR spectroscopy Mass spectrometry Cryo-electron microscopy Proteomics Western blot Immunoprecipitation ELISA Flow cytometry
Related	Gene expression Protein biosynthesis Post-translational modification Protein domain Protein structure database Protein–protein interaction prediction Protein disorder Protein function prediction
This protein-related article is a stub.

HNCA experiment

Principle[edit | edit source]

Procedure[edit | edit source]

Applications[edit | edit source]

See also[edit | edit source]

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