Ancestim
Ancestim is a recombinant methionyl human stem cell factor, which has been commercialized under the brand name StemGen by Amgen. This therapeutic protein was developed with the intent of aiding in stem cell mobilization, particularly in the context of hematological malignancies and disorders where stem cell transplantation is a treatment avenue.
Production and Molecular Characteristics[edit | edit source]
Ancestim is synthesized using E. coli bacteria that have been genetically modified to express the human stem cell factor. Here's a deeper look into its molecular details:
- Protein Length: Ancestim is constructed of 166 amino acids, bestowing it a distinctive molecular structure.
- Molecular Weight: With a monomeric molecular weight of approximately 18,500 daltons, Ancestim in its physiological environment often assembles as a non-covalently associated dimer.
- Amino Acid Sequence: It reproduces the amino acid sequence expected from human DNA sequence analysis, barring the retention of an N-terminal methionine residue resulting from its expression in E. coli.
- Glycosylation: Being produced in E. coli, Ancestim is devoid of glycosylation, implying it doesn't contain the sugar moieties frequently found in proteins expressed in mammalian cells[1].
Therapeutic Uses[edit | edit source]
Ancestim's application is rooted in oncology, specifically in the mobilization of peripheral hematopoietic stem cells—a procedure pivotal for patients diagnosed with cancers that are treated via stem cell transplantation:
- Formulation: Delivered as a sterile, white, lyophilized powder, Ancestim is free from preservatives. Post-reconstitution, it's primed for administration.
- Route of Administration: Once reconstituted, Ancestim is administered subcutaneously.
- Combination Therapy: Often, Ancestim is paired with filgrastim to boost the efficiency of stem cell mobilization[2].
Conclusion[edit | edit source]
The development and deployment of Ancestim accentuate the marvels of genetic engineering in pioneering innovative therapeutics. Harnessing the potent protein expression system of E. coli to yield a crucial human stem cell factor, Ancestim stands as a testament to the progress of modern medicine. In the continually evolving field of oncology, agents like Ancestim, pivotal for stem cell mobilization and transplantation, undeniably hold significant importance.
References[edit | edit source]
- ↑ Apweiler, R., Hermjakob, H., & Sharon, N. (1999). On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochimica et Biophysica Acta (BBA) - General Subjects, 1473(1), 4-8.
- ↑ Duarte, R. F., Shaw, B. E., Marín, P., Kottaridis, P., Ortiz, M., Morante, C., ... & Sureda, A. (2015). Plerixafor plus granulocyte CSF can mobilize hematopoietic stem cells from multiple myeloma and lymphoma patients failing previous mobilization attempts: EU compassionate use data. Bone Marrow Transplantation, 50(6), 812-817.
| Ancestim Resources | |
|---|---|
|
 |
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
|
WikiMD's Wellness Encyclopedia |
| Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD
